This lecture will cover key steps for the cruelty investigator when responding to an animal cruelty case. Topics include crime scene analysis, evidence recognition, forensic testing, blood stain pattern analysis, and the use of thermal imaging. The findings in the most common types of cruelty will be presented including supportive diagnostic testing available. Critical documentation and actions by first responders will be discussed. Resources for forensic testing and further training will be included. Cases will be presented throughout highlighting the use of forensics and working with veterinarians.